WORLD OF REPRODUCTIVE BIOLOGY: DNA Transfer Technique Honed for Mitochondrial Disease

Mitochondrial DNA disease and developmental implications for reproductive strategies

Mitochondrial diseases are potentially severe, incurable diseases resulting from dysfunctional mitochondria. Several important mitochondrial diseases are caused by mutations in mitochondrial DNA (mtDNA), the genetic material contained within mitochondria, which is maternally inherited. Classical and modern therapeutic approaches exist to address the inheritance of mtDNA disease, but are potenti...

Mitochondrial transfer: Implications for assisted reproductive technologies

The use of mitochondrial transfer as a clinic procedure is drawing closer to reality. Here we provide a detailed overview of mitochondrial transfer techniques - both established and recent - including pronuclear, spindle, ooplasmic and blastomere transfer. Reasons as to why some techniques are more suitable for the prevention of mitochondrial DNA disease than others, as well as the advantages a...

A REPORT OF RAPID AND EFFICIENT METHOD FOR MITOCHONDRIAL DNA

Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...

The biology of mitochondrial disease.

Plant Mitochondrial Electron Transfer and Molecular Biology.

Aerobic respiration, a process performed by all plants, involves the controlled oxidation of metabolites containing reduced carbon to produce carbon dioxide and water as the final products (Taiz and Zeiger, 1991). Severa1 types of reduced carbon compounds, including fatty acids, organic acids, and amino acids, can serve as the primary reducing substrates for plant respiration. However, the most...